Journal: Clinical Science

Article Title: Relaxin improves multiple markers of wound healing and ameliorates the disturbed healing pattern of genetically diabetic mice

doi: 10.1042/cs20130105

Figure Lengend Snippet: Figure 7 VEGFR-2 and VE-cadherin staining (A, B) Representative immunohistochemical VEGFR-2 staining of skin wounds samples from diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or RLX (25 μg/mouse per day, subcutaneously) at day 12. Arrows indicate staining positivity. (C, D) Representative immunohistochemical VE-cadherin staining of skin wounds samples from diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or RLX (25 μg/mouse per day, subcutaneously) at day 12. Arrows indicate staining positivity. (C, F) Quantification of VEGFR-2- and VE-Cadherin-positive cells per field in normoglycaemic (db + / + m) and diabetic (db + /db + ) mice treated with vehicle (6 μl of 0.9 % NaCl/mouse per day) or with RLX (25 μg/mouse per day, subcutaneously) at day 12. Each bar represents the mean +−S.D. of six animals. *P < 0.05 compared with db + / + m + vehicle; §P < 0.01 compared with db + / + m + vehicle; #P < 0.01 compared with db + /db + + vehicle.

Article Snippet: Slides were incubated overnight with primary antibody to detect CD31, CD34, MMP-11, VE-cadherin, VEGFR-1 (all from Abcam) and VEGFR-2 (Cell Signaling) as described previously [20].

Techniques: Staining, Immunohistochemical staining